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Nucleic Acid Processors, Replication, Thermal Cycle

Definition : Nucleic acid replication processors that perform nucleic amplification (replication) following a thermal cycle. The most commonly used procedure is polymerase chain reaction, which is performed in a vial containing the template molecule (the DNA or RNA to be copied) and two primer molecules to start the copying process. The primers are short chains of the four different chemical components that make up any strand of genetic material. In the first part of the process, the two DNA chains in the double helix are separated at about 90 degrees Celcius (C) (195 degrees Farenheit (F)). The vial is cooled to 55 degrees C (131 degrees F) to bind (anneal) the primers to the end of the DNA strands. In the final step of the reaction a complete copy of the templates is made at around 75 degrees C (158 degrees F). The three steps take less than two minutes. At the end of the cycle, each piece of DNA in the vial has been duplicated. Repeating the process 30 or more times may yield more than one billion copies.

Entry Terms : "Polymerase Chain Reaction (PCR) Replicators" , "Polymerase Chain Reaction (PCR) Amplifiers"

UMDC code : 20811

   

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Abbott Molecular Inc

Abbott's rapidly growing molecular diagnostics business provides physicians with critical information based on the early detection of pathogens and subtle changes in patients' genes and chromosomes, allowing for earlier diagnosis, selection of appropriate therapies and monitoring of disease progression. The business includes instruments and reagents used to conduct sophisticated analysis of patient DNA and RNA.

Agilent Technologies Inc

As the world's premier measurement company, Agilent offers the broadest range of innovative measurement solutions in the industry. The company's four businesses – Chemical Analysis, Life Sciences, Diagnostics and Genomics, and Electronic Measurement -- provide customers with products and services that make a real difference in the lives of people everywhere. And at Agilent Research Laboratories, we conduct research that anticipates customer needs and produces breakthroughs that power growth.

Anachemia Science

Anachemia Science

Applied Biosystems

Our newly expanded portfolio of market-leading lab services can provide your high-performance lab with a comprehensive suite of services, or just the ones you want. From business consulting and protocol development, to instrument repair and optimization, our services can help you increase your lab's productivity and efficiency.

Applied Biosystems (Canada)

Applied Biosystems (Canada)

Bio-Rad (France)

Bio-Rad (France)

Bio-Rad Hungary Kft

Bio-Rad Hungary Kft

Bio-Rad Laboratories (Singapore) Pte Ltd

Bio-Rad Laboratories (Singapore) Pte Ltd

Bio-Rad Laboratories AG

Bio-Rad Laboratories AG

Bio-Rad Laboratories bv

Bio-Rad Laboratories bv

Bio-Rad Laboratories Ges mbH

Bio-Rad Laboratories Ges mbH

Bio-Rad Laboratories GmbH

Bio-Rad Laboratories GmbH

Bio-Rad Laboratories Inc

Bio-Rad Laboratories continues to play a leading role in the advancement of scientific discovery by offering a broad range of innovative tools and services to the life science research and clinical diagnostics markets. Founded in 1952, Bio-Rad has a global team of more than 7,000 employees and serves more than 100,000 research and industry customers worldwide through the company's global network of operations.

Bio-Rad Laboratories Inc

Bio-Rad Laboratories continues to play a leading role in the advancement of scientific discovery by offering a broad range of innovative tools and services to the life science research and clinical diagnostics markets. Founded in 1952, Bio-Rad has a global team of more than 7,000 employees and serves more than 100,000 research and industry customers worldwide through the company's global network of operations.

Bio-Rad Laboratories Ltd

Bio-Rad Laboratories Ltd

Bio-Rad Laboratories Ltd (UK)

Bio-Rad Laboratories Ltd (UK)

Bio-Rad Laboratories Pty Ltd

Bio-Rad Laboratories Pty Ltd

Bio-Rad Laboratories srl

Bio-Rad Laboratories srl

Bio-Rad Pacific Ltd

Bio-Rad Pacific Ltd

Bio-Rad SA-NV (Belgium)

Bio-Rad SA-NV (Belgium)

Bio-Rad Thailand

Bio-Rad Thailand

Cepheid

Cepheid (Nasdaq: CPHD) is a leading molecular diagnostics company that is dedicated to improving healthcare by developing, manufacturing, and marketing accurate yet easy-to-use molecular systems and tests. By automating highly complex and time-consuming manual procedures, the company's solutions deliver a better way for institutions of any size to perform sophisticated genetic testing for organisms and genetic-based diseases.

DiaMed-Caribbean Inc

DiaMed GmbH is a subsidiary of Bio-Rad Laboratories, Inc., based in Hercules, CA. It specializes in research and development, production and worldwide services for laboratory diagnostic products.

Eppendorf North America Inc

With headquarters in New York and over 200 employees, we are the largest subsidiary of Eppendorf AG (Hamburg, Germany)—world-renown manufacturer of the highest quality laboratory instruments and consumables for the life sciences.

Fisher Healthcare

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries.

Fisher Scientific Co (Canada)

Fisher Scientific Co (Canada)

Fisher Scientific GmbH

Fisher Scientific GmbH

Fisher Scientific Oy

Fisher Scientific Oy

Fisher Scientific UK

Fisher Scientific UK

Idaho Technology Inc

The Company currently holds over 70 patents related to polymerase chain reaction (PCR), including rapid PCR cycling. The Company has used its extensive patent portfolio to successfully market nearly 200 products to the clinical, research and military markets. The Company manufactures and distributes its proprietary diagnostic respiratory panel, FilmArray RP, which operates on its user-friendly FilmArray system, to hospital-based clinical laboratories in the U.S. and E.U.

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